Table 1 of
Warburton, Mol Vis 2005;
11:1122-1134.Table 1 of
Warburton, Mol Vis 2005;
11:1122-1134.
Table 1. Proteins identified in retinal lipofuscin
These proteins were identified from in-gel digestion followed by mass spectrometry from the respective SDS-PAGE gel regions or 2D gel spots. These proteins demonstrate that RLF contains components of photoreceptor disks and of lysosomes. Furthermore, 1D- and 2D-based protein identification is complementary, with 1D-based methods identifying significantly more of the heterogeneous and hydrophobic proteins that are found in RLF. We could not differentiate between creatine kinase and arginine kinase because of identical peptide sequences. KIAA0617 was only identified from one peptide with relatively low confidence. Items marked with an asterisk (*) were previosly reported in Schutt et al. [11].
Gel area/
Protein NCBI spot number Tissue specificity Subcellular location Alternate names Gene ontology
----------------------- -------- --------------- ---------------------------- ------------------------ ----------------------- -----------------------------------
1D gel system
4F2 antigen heavy chain 177207 D2 type II membrane protein CD98 antigen calcium ion/amino acid transport
11-cis retinol 2492753 I retinal pigment epithelium membrane-associated retinol dehydrogenase visual perception
dehydrogenase
acyl-CoA dehydrogenase 3273228 F mitochondrial inner fatty acid beta-oxidation
membrane
alpha 1-antitrypsin 24438 H plasma extracellular alpha-1-antiproteinase endopeptidase inhibitor activity
alpha-tubulin 4 32015 A, D2 protein binding
alpha-tubulin 1* 37492 F protein binding
anion transport protein 28714 D1 erythrocytes integral membrane AE 1, CD233 antigen cell ion homeostasis
protein
annexin A2* 18645167 A membrane associated lipocortin II calcium ion binding
beta-globin* 29446 B erythrocytes hemoglobin beta chain oxygen transport activity
calnexin 2134858 E1 type I membrane protein calcium ion binding
cathepsin D* 494296 B lysosomal lysosome
ceroid-lipofuscinosis 5729770 H lysosomal tripeptidyl-peptidase I tripeptidyl-peptidase I
cytochrome c oxidase II 14016 L integral membrane cytochrome c oxidase cytochrome-c oxidase activity
subunit protein, mitochondrial
inner membrane
interphotoreceptor 33961 D1 interphotoreceptor matrix interstitial lipid metabolism, visual peception
retinoid-binding retinol-binding protein
protein
keratin 30154839 B, F, M cytoskeleton organization
lysosomal integral 1362856 E1 type II membrane LIMP II, CD36 antigen integral to plasma membrane
membrane protein II protein, lysosomal like 2
myelin protein zero 127721 A, B, D1/2, peripheral nervous system type I membrane protein structural molecule activity,
E1/2, F, I, J, schwann cells synaptic transmission
L
NADH-ubiquinone 128826 E1 mitrochondrial inner NADH dehydrogenase electron carrier activity
oxidoreductase membrane
Na+/K+-exchanging 88214 D1 integral membrane sodium pump 3 ATP hydrolysis, proton transport
ATPase protein
peripherin 4506465 A photoreceptor rod rim region integral membrane retinal degeneration visual perception
protein slow protein
phospholipase C-alpha 303618 F endoplasmic reticulum protein disulfide signal transduction
isomerase A3
rhodopsin 4506527 D1/2, E1/2, photoreceptor rod integral membrane Opsin 2 phototransduction,
F, G, H, I protein G-protein coupled receptor activity
rod outer segment 4506575 A, D2, H, I photoreceptor rod rim region integral membrane ROSP1 visual perception
membrane protein I protein
retinal pigment 4506591 D2, E1/2 retinal pigment epithelium RPE65 visual perception
epithelium-specific
protein
retinal S-antigen* 4506781 F, G retina and pineal gland rod photoreceptor signal transduction
arrestin
serum albumin* 28592 A plasma secreted body fluid osmoregulation
transducin 31865 A photoreceptor rod membrane associated visual perception
trypsin 136429 A, D1/2, extracellular protease
E1/2, F, G, H,
I, M
2D gel system
ATP synthase, beta 32189394 19, 47, 71 mitochondria, membrane tranpsorter activity
subunit* protein
calnexin 2134858 3 type I membrane protein, calcium ion binding
ER
calreticulin* 4757900 6 endoplasmic reticulum protein folding
lumen
calumenin 2809324 16 ubiquitously expressed endoplasmic retic. crocalbin calcium ion binding
lumen/secreted
creatine kinase/ 180570 74 cytoplasmic creatine kinase activity
arginine kinase*#
cathepsin D* 494296 37,38 lysosomal
ceroid-lipofuscinosis, 5729770 21,24 lysosomal
neuronal 2
dnaK-type molecular 87528 4,5,52 endoplasmic reticulum
chaperone HSPA5 lumen
heat shock protein 15010550 7 endoplasmic reticulum GRP94, tumor rejection response to unfolded protein
gp96a lumen antigen
KIAA0908 4240305 57 ubiquitous lysosomal prenylcysteine oxidase lysosome, lyase activity
KIAA0617 40788303 30 widely expressed nuclear and golgi cell cycle arrest
motor protein* 516764 74 mitochondrial inner mito. inner memb. mitochondrial inner membrane
membrane protein, mitofilin
mutant β-actin 28336 27 cytoplasmic acetyltransferase complex
NADH-ubiquinone 128826 1, 2, 32, 33, mitrochondrial inner NADH dehydrogenase electron carrier activity
oxidoreductase 41, 54 membrane
phospholipase c-α 303618 11 endoplasmic reticulum signal transduction
pre-mRNA splicing 338043 32 mitochondrial matrix hyaluronan-binding immune response
factor SF2p32 protein 1
prohibitin* 4505773 39 widely expressed cytoplasmic regulation of cell cycle
protein disulfide 5031973 7,12,19 endoplasmic reticulum
isomerase lumen
vimentin* 4507895 17 highly expressed in structural constituent
fibroblasts
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