Figure 4 of
Liu, Mol Vis 2005;
Figure 4. C3-induced changes in actin cytoskeleton and focal adhesions in human trabecular meshwork cells
Human trabecular meshwork cells were transduced with AdGFP or AdC3GFP for 4 days. A,D: No virus. B,E: AdGFP-transduced. C,F: AdC3GFP-transduced. Cells were labeled with phalloidin (A-C) or anti-vinculin monoclonal antibody (D-F). Note abundant stress fibers in cells exposed to no virus (A) or transduced with AdGFP (B) which contrasts with cells transduced with AdC3GFP (C). Vinculin labeling (arrows) was associated with focal adhesions and cell-cell junctions. Note that AdC3GFP-transduced cells (F) are essentially devoid of vinculin labeling. The scale bar represents 50 μm.