Table 1 of
Gomez-Cabrero, Mol Vis 2005;
11:1071-1082.Table 1 of
Gomez-Cabrero, Mol Vis 2005;
11:1071-1082.
Table 1. Oligonucleotides used to modify the pRSETA vector
The nucleotides marked in red indicate the NheI sites. The nucleotides marked in blue indicate the XhoI sites. The nucleotides marked in brown indicate the EcoRI site. The nucleotides marked in orange indicate the HindIII site. The nucleotides marked in green indicate the transduction sequence PTD4. In oligonucleotide bGalXC-F and XhoI-Profilin, the nucleotides marked in purple indicate the first codon. In oligonucleotide bGalSE-R and Profilin-HindIII, the nucleotides marked in turquoise indicate the last codon.The asterisk indicates a compatible end.
Oligonucleotide Sequence (5'-3')
----------------- --------------------------------------------
pre-PTD4-MCS-F CTAGCATGACTGGTGGACAGCAAATGTGTACAGGTGGTTACGCT
NheI* GlyGly
CGGGCTGCTGCTCGGCAGGCTCGGGCTGGTGGTGGTGGTC
PTD4 GlyGlyGlyGly XhoI*
pre-PTD4-MCS-R TCGAGACCACCACCACCAGCCCGAGCCTGCCGAGCAGCAGCCCG
XhoI*GlyGlyGlyGly PTD4
AGCGTAACCACCTGTACACATTTGCTGTCCACCAGTCATG
GlyGly NheI*
pre-MCSnoPTD4-F CTAGCATGACTGGTGGACAGCAAATGACGCGTC
NheI* XhoI*
pre-MCSnoPTD4-R TCGAGACGCGTCATTTGCTGTCCACCAGTCATG
XhoI* NheI*
bGalXC-F ATCTATCTCGAGCCCGTCGTTTTACAAC
XhoI beta-Gal first codon
bGalSE-R TAAATGAATTCCTTTTTGACACCAGACCAAC
EcoRI beta-Gal last codon
XhoI-Profilin TAATAACCGCTCGAGATGGCCGGG
XhoI Profilin first codon
Profilin-HindIII CCCCCAAGCTTGGGGTACTGGGAAC
HindIII Profilin last codon
(nt 556-554 in NM_005022)
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