Table 4 of
Gupta, Mol Vis 2005;
11:1018-1040.
Table 4. RT-PCR: Confirmation of microarray findings
Fold changes (±standard error of the mean), normalized to β-actin, of genes from dexamethasone compared to vehicle treated HLE B-3 cells analyzed by microarray were verified by RT-PCR from HLE B-3 cells and RNA from a pair of treated cultured donor lenses. Data were quantitated by crossing point analysis of SYBR green fluorescence. ANOVA was used to calculate p values. Genomic DNA sequences for all the genes listed were examined and found to contain putative GREs.
Microarray Real time ----------------------- ----------------------- Gene B-3 p value B-3 p value Donor ------ ------------- ------- ------------- ------- ----- 4 h PER 1 6.01 ± 0.57 0.03 6.36 ± 0.98 0.006 3.95 DSIP 5.56 ± 0.17 0.0005 13.6 ± 0.55 0.0004 5.49 HSP70 4.84 ± 0.93 0.06 1.42 ± 0.31 0.7 1.49 PRKAR1 4.37 ± 0.94 0.09 1.51 ± 0.17 0.04 1.32 F2R 4.00 ± 0.55 0.01 1.66 ± 0.03 0.004 2.20 PAI-1 3.5 ± 0.33 0.002 6.42 ± 0.61 0.0003 1.50 GADD45 2.76 ± 0.88 0.7 1.43 ± 0.11 0.04 1.00 SGK 2.41 ± 0.097 0.003 2.86 ± 0.14 0.0004 3.60 PHLDA -1.62 ± 0.06 0.02 -1.40 ± 0.012 0.03 -2.80 IER3 -2.05 ± 0.15 0.01 -1.77 ± 0.12 0.01 -1.69 NGF -2.19 ± 0.12 0.01 -1.81 ± 0.007 0.005 -3.01 MCP-1 -5.76 ± 0.59 0.0008 -6.72 ± 0.037 0.03 -2.01 16 h SCNN1A 11.94 ± 0.17 0.002 12.04 ± 0.12 0.0001 DSIP 5.97 ± 0.047 0.0002 9.20 ± 0.16 0.001 PER1 4.59 ± 0.12 0.008 5.90 ± 0.42 0.02 DUSP-1 3.35 ± 0.043 0.00007 4.40 ± 0.26 0.03 PAI-1 3.01 ± 0.070 0.001 3.56 ± 0.33 0.04 F2R 1.15 ± 0.30 0.08 1.55 ± 0.033 0.3 CCND1 -1.61 ± 0.13 0.003 -1.60 ± 0.083 0.02 PHLDA -1.81 ± 0.13 0.0002 -1.7 ± 0.30 0.02 CCL2 -2.03 ± 0.09 0.006 -2.3 ± 0.32 0.05 CCK -2.16 ± 0.076 0.003 -2.40 ± 0.23 0.02 |