Table 2 of
Otteson, Mol Vis 2005;
11:986-995.
Table 2. Phylogenetic comparison of crx exon-intron splice boundaries
Alignment of splice junctions of zebrafish crx with human and mouse CRX reveals conservation of splice acceptors and donor sites located within the coding regions. The identified 5' UTR in zebrafish crx is contained entirely in exon 1, whereas the 5' UTR in mouse and human crx is divided into two exons. Despite this difference, the positions of the splice junctions in coding regions are highly conserved. The splice junction between exons 1 and 2 in zebrafish crx is located at the same position, relative to the ATG initiation codon, as the splice junctions between exons 2 and 3 in human and mouse. Splice junctions were identified by aligning cDNA sequences with the corresponding genomes using the BLAT program at the UCSC genome database (UCSC). The human sequence (BC016664) marked with an asterisk (*) is aligned with the human genome May 2004 assembly; the mouse sequence (AK053533) marked with a double asterisk (**) is aligned with the mouse genome October 2003 assembly; zebrafish (AF503443) is aligned with the zebrafish genome June 2004 assembly. There are additional splice-variants unique to human crx [28].
Exon Intron size size Species Exon Acceptor splice site (bp) Donor splice site (bp) ---------- ---- -------------------------- ---- ------------------------- ------ human* 1 >103 TTCTGAAGgtgagcgtc 12392 mouse** 1 >78 TTCCAAAGgtgagtctt 8540 zebrafish 1 >515 ATG(n90)CTACCCAGgtcagttca 731 human 2 tctcttgcagGCCCCCTG 135 ATG(n90)CTACCCAAgtgagtaca 859 mouse 2 tctcttgtagGTGCCCTC 135 ATG(n90)ATACTCAAgtaagtatg 1633 zebrafish 2 gtatgtatagCCACTCCG 152 GAGTTCAGgtgagtcct 583 human 3 cccaccccagGCGCCCCC 152 GGGTTCAGgtggggtgg 1698 mouse 3 ccccgcttagGTGCCCCT 152 GGGTCCAGgtaggatga 953 zebrafish 3 gtttcttcagGTGTGGTT(n586)TGA 1725 human 4 tatcccccagGTTTGGTT(n637)TGA 1829 mouse 4 ctctatctagGTCTGGTT(n637)TGA 2478 |