Table 1 of Otteson, Mol Vis 2005; 11:986-995.


Table 1. Primers for PCR amplification of zebrafish crx from genomic DNA

Oligonucleotide primers used for PCR amplification of zebrafish crx for sequence analysis were designed using Primer3 (Primer3) or Vector NTI (Invitrogen Corp.) software and the published zebrafish crx cDNA sequence (GenBank accession number AF503443) [4] and obtained from IDT as standard, desalted oligonucleotides (25 nmole synthesis). Annealing temperatures were optimized using a gradient program on a MJ Research DNA Engine thermocycler (BioRad).

                                                                         Temp
                                                                       (anneal)     Size
 Forward primer (5'-3')       Reverse primer (5'-3')        Target       (°C)       (bp)
-------------------------   ---------------------------   ----------   --------   ---------
CTCATAGTGTCCCGCTCCTGTCC     GCTCTCTCTCTCCCTCCCTCTCTCAC    exon 1:5'       60         364
TTCTCGCATATAGACATTTATTTG    ATAAAACGTCTAACAGAAACACCATTA   exon 1:3'       55         471
AACCTCCTGAATTGTTTTAAGCTG    TGTTGAGAAGGATGTGTGAGAGGC      exon 2          55         242
ATCCATCCATTCATCCATCTAACAC   CTCTCTCACTTTCTTTTCTACAGCAC    exon 3:5'       55         990
ACGGTCAGCCCTCTTCCTACAGCC    GGAAGGCTCAGATATAGGGGTGG       exon 3:mid      60         725
GGAAGGAAAGATGCGTGAAGAAG     AACCTTCAGCTCCCCTCTGTACGTG     exon 3:3'       60         933
TTCTCGCATATAGACATTTATTTG    TGTCTGGATAGCGTGTTTTGGTGA      intron 1        62        1243
ACCACCTTCACTCGCACCCAG       TGTAGGAAGAGGGCTGACCGTAGC      intron 2        62      1026-1064
AACCTGCCAGAGGGTTACT         TCAACAGATGGCTTCAGTGC          Z13833          50      177 [33]

Otteson, Mol Vis 2005; 11:986-995 <http://www.molvis.org/molvis/v11/a118/>
©2005 Molecular Vision <http://www.molvis.org/molvis/>
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