Table 1 of
Otteson, Mol Vis 2005;
11:986-995.Table 1 of
Otteson, Mol Vis 2005;
11:986-995.
Table 1. Primers for PCR amplification of zebrafish crx from genomic DNA
Oligonucleotide primers used for PCR amplification of zebrafish crx for sequence analysis were designed using Primer3 (Primer3) or Vector NTI (Invitrogen Corp.) software and the published zebrafish crx cDNA sequence (GenBank accession number AF503443) [4] and obtained from IDT as standard, desalted oligonucleotides (25 nmole synthesis). Annealing temperatures were optimized using a gradient program on a MJ Research DNA Engine thermocycler (BioRad).
Temp
(anneal) Size
Forward primer (5'-3') Reverse primer (5'-3') Target (°C) (bp)
------------------------- --------------------------- ---------- -------- ---------
CTCATAGTGTCCCGCTCCTGTCC GCTCTCTCTCTCCCTCCCTCTCTCAC exon 1:5' 60 364
TTCTCGCATATAGACATTTATTTG ATAAAACGTCTAACAGAAACACCATTA exon 1:3' 55 471
AACCTCCTGAATTGTTTTAAGCTG TGTTGAGAAGGATGTGTGAGAGGC exon 2 55 242
ATCCATCCATTCATCCATCTAACAC CTCTCTCACTTTCTTTTCTACAGCAC exon 3:5' 55 990
ACGGTCAGCCCTCTTCCTACAGCC GGAAGGCTCAGATATAGGGGTGG exon 3:mid 60 725
GGAAGGAAAGATGCGTGAAGAAG AACCTTCAGCTCCCCTCTGTACGTG exon 3:3' 60 933
TTCTCGCATATAGACATTTATTTG TGTCTGGATAGCGTGTTTTGGTGA intron 1 62 1243
ACCACCTTCACTCGCACCCAG TGTAGGAAGAGGGCTGACCGTAGC intron 2 62 1026-1064
AACCTGCCAGAGGGTTACT TCAACAGATGGCTTCAGTGC Z13833 50 177 [33]
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