Table 1 of
Zhang, Mol Vis 2005;
11:977-985.Table 1 of
Zhang, Mol Vis 2005;
11:977-985.
Table 1. Primers for sequencing and deletion detection
Primers for sequencing and deletion detection. Primers were selected using the PrimerSelect program of the Lasergene package (DNASTAR, Madison, WI). The number following the "E" in the primer name indicates the exon region of the GRK1 (RHOK) gene; the forward (F) and reverse (R) primers are indicated in the last character of the name. Length of the PCR products for exon RHOK-E4 is approximate as the whole genomic sequence around exon 4 is incomplete in the human genome database.
PCR
Tm length
Name Sequence (5'-3') (°C) (bp)
--------- ---------------------------- ---- ------
RHOK-E1AF CAGGCCAAGGGCAGCAGTCAGG 61.8 497
RHOK-E1AR TTCGCCACTATCCCCTCATCCA
RHOK-E1BF CGGCCCTGGAGCTCTGGAAAGA 63.5 551
RHOK-E1BR TAAGGGCCCCGGAGCCGACAGT
RHOK-E2F TGCGACTGCTCCGTGGCTGTG 60.6 394
RHOK-E2R AGCGAGAAGGGGATTGTGATGG
RHOK-E3F TGGCCTTCGGGTGTCCTCTGC 62 436
RHOK-E3R ATGAACCCCCACCGTCCACAC
RHOK-E4F CGCCTGTCCTGTGCAGCCAG 63 150
RHOK-E4R GTAACCCATGCACGGAGCCT
RHOK-E5F GCATCCCCAGAGCATCAGTCC 61 339
RHOK-E5R CCACCCCAGGGCCCACAGAACAA
RHOK-E6AF GAGGCGGGTCTGGCAGGGCTAAG 63.6 387
RHOK-E6AR GGGCCTTGCTGGGGGCAGAG
RHOK-E6BF TTCTGCGAGGCGCTGCTGGAGA 62.7 524
RHOK-E6BR GCGCACATAGTATCTTCCGCATCC
RHOK-E7F CCATGGGGGAGGGGGCTTTTTG 62.8 519
RHOK-E7R GGAGATGGCGTGATGGGGACCTG
RHOK-D1 GTGGTGCTGTGGGGGACGTGTGATGC
RHOK-D2 AGGCGGAGGGGAGGGGACATTGGGAAGT
RHOK-D5 TGACGGTGGAGGGCGCAGGGTGTAG
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