Table 1 of
Gu, Mol Vis 2005;
11:971-976.Table 1 of
Gu, Mol Vis 2005;
11:971-976.
Table 1. PCR primers and product sizes
Gene specific PCR primer sequences for all exons and flanking introns sequences of CRYGA, CRYGB, CRYGC, and CRYGD and the size of each PCR product.
Fragment
Gene Exon Strand Sequence (5'-3') size (bp)
----- ---- ------- ---------------------- ---------
CRYGA 1 Forward GTCAGCTGGAAGGAACATCC 291
Reverse TGAGGACAACTCGAAAATGC
2 Forward GGGTCAGGCCTTGCTATTCT 450
Reverse CCATGTCTATTGGGGGTCTG
3 Forward GGATTGATTGAACCTGGGAG 659
Reverse GGTGAAAGTTGCAGTGAGCA
CRYGB 1,2 Forward GGTGGTGCATGCCTGTAA 679
Reverse GCCCTTTTGTGTGATTTCCT
3 Forward AAACTTGGCCTGGGAGAACT 553
Reverse GCTTCCCATCATGAAAACAT
CRYGC 1 Forward CATTTCCAGTGAATGCAGGA 442
Reverse CGCAGCAAGTATTGTTGACC
2 Forward GGAAGGTGAGCAGAACACAA 476
Reverse TGGCTTATTCAGGTCTCTGATG
3 Forward AATGACAATTCCATGCCACA 534
Reverse CCCACCCCATTCACTTCTTA
CRYGD 1,2 Forward CTTATGTGGGGAGCAAACT 620
Reverse CAGCAGCCCTCCTGCTAT
3 Forward GAAACAGCTATGACCATGCACA
CTTGCTTTTCTTCTCTTT 435
Reverse ATACGACTCACTATAGGGCAAG
ACACAAGCAAATCAGTGCC
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