Table 1 of
Motallebipour, Mol Vis 2005;
11:950-957.Table 1 of
Motallebipour, Mol Vis 2005;
11:950-957.
Table 1. Sequence of primers used for study of polymorphisms
The table below summarizes all primers used in the study of polymorphisms for PCR, sequencing, and SNaPshotTM. The column "Primer name" refers to the type of polymorphism and "Position" is its location in relation to transcriptional start position at +1. The sequence for the CCTTT-microsatellite primer was taken from [25]. For SNaPshotTM only one primer is required for each SNP. In the "Sequence" column for the SNaPshotTM primers, the numbers in parentheses indicate the number of "GATC" repeats.
Primer name Direction Position Sequence (5'-3')
-------------- --------- -------- -------------------------------
PCR primers
C/T Forward -3761 TCCAGGATAATGAGCCCAAG
Reverse GTCCCACCACAAACACCTG
C/T Forward -3031 AAAAACCATTGCATTTTACACTTT
Reverse TGGCAAGCTGTGAGAAGGTA
C/G Forward -2441 GCTCTCTGCAGCCTCTAACTC
Reverse AGCCAGCTGGGAAGGAAATA
C/T Forward -1659 TCTTGGGTGGGGGCATTA
Reverse CATCTTCCCTTCAGGCTTCA
G/T Forward -1026 TCCCTTTGCTTCTCAACTTCTC
Reverse TGGAGTCTCATTCTGTCACCA
A/G Forward -277 AGCTTCCTGGACTCCTGTCA
Reverse GGAGCCTCAGTTTTCGACTC
C/T Forward 1211 GATCTCAGATTTCGAGGGATGA
Reverse ATGGCTTTACAAAGCAGGTCA
C/T Forward 1638 GATCTCAGATTTCGAGGGATGA
Reverse ATGGCTTTACAAAGCAGGTCA
Sequencing primers
CCTTT- Forward -2600 ACCCCTGGAAGCCTACAACTGCAT
microsatellite Reverse GCCACTGCACCCTAGCCTGTCTCA
TAAA-repeat Forward -751 ACTCCGCTCCAGTCTTGGT
Reverse AGGAAGGCTATTGGCACAGA
GTGTGTT Forward -453 TCTGTGCCAATAGCCTTCCT
ins/del Reverse TCCCTGTCCATCCTCTCACT
SNaPshot primers
C/T Forward -3761 ATCAGAAGAGGCCAGGAATG
C/T Forward -3031 (GATC)(2)CAGTGAAGCTGTGCCTCGAA
C/G Reverse -2441 (GATC)(4)GAAAATAAATTAGCTGGGTATG
C/T Forward -1659 (GATC)(6)TTCCTTGAACAAGGCAGAAC
G/T Reverse -1026 (GATC)(3)GGATTACAAGGGTTAGCCAC
A/G Forward -277 (GATC)(2)GGCTCCGTGGTGCCTCT
C/T Forward 1211 (GATC)(7)TTGAGTGCTTTCCCCAGTAA
C/T Forward 1638 (GATC)(10)CCTTCCCTCACCTCTGCTTC
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