Figure 1 of
Motallebipour, Mol Vis 2005;
11:950-957.Figure 1 of
Motallebipour, Mol Vis 2005;
11:950-957.
Figure 1. Specific protein binding by the DS (CCTTT)14 allele of the microsatellite in the iNOS promoter
An EMSA was performed with Klenow-fragment treated, labeled, double stranded (CCTTT)14 oligonucleotide, incubated with HeLa nuclear extract to examine nuclear factor binding capability of the microsatellite. Lane 1: Negative reaction containing labeled DS (CCTTT)14 oligonucleotide. Lane 2: Positive reaction with labeled DS (CCTTT)14 oligonucleotide and 1.5 μl of HeLa nuclear extract (concentration of stock >2.4 μg/μl). Lanes 3-5: Specific competitor reaction with same components as Lane 2 with 10x, 25x, and 50x excess of unlabeled DS (CCTTT)14 oligonucleotide added. Lane 6: A competitor reaction with 50x excess of unlabeled Sp1 oligonucleotide. Lanes 7-9: Supershift with 0.2 μg of antibody against PTB, hnRNPK, and Ap2.
