Table 1 of
Bolz, Mol Vis 2005;
11:929-933.Table 1 of
Bolz, Mol Vis 2005;
11:929-933.
Table 1. PCDH21 primers (exons and marker)
The length of coding sequences and the size of the genomic amplicon are given for each exon. For IVS9-CA, the expected size range is given. Besides the primer sequences for the amplifications of the 17 PCDH21 exons, the length of coding sequences and the size of the genomic amplicons is given. In the 5' and 3' prime exon-intron boundary sequences, uppercase letters indicate exonic sequence and lowercase letters indicate intronic sequence. For the polymorphic CA-repeat sequence in intron 9 (IVS9-CA), the expected size range is given.
5' and 3'
Size exon-intron Amplicon
Exon (bp) boundaries Primers (5'-3') (bp)
---- ---- -------------- ---------------------------- --------
1 55 cggagacATGAGGC F: ggcaggagcagatccgagccgtg 364
TGCTTGGgtgagtg R: gtcccactctggaagtggtccagg
2 96 tttccagCTCAGGC F: gctggtgcgaagctccctcctgg 357
CCTGTAGgtgagta R: gccagacttaagtcgccatggcag
3 146 cttccagGCTCTCA F: gccagcaatgagcaccaagttcagg 381
CAGAGAGgtatggg R: ccaatgtgctccaggggaacttcag
4 51 ttcacagAGGGAAG F: gcatgctgtcaccaccacttaccc 286
GAATCTGgtgagtg R: gggaggaatcgtgccagcctcacc
5 90 ttctcagGTGGCCG F: ggcagagacgtgtacattggagtg 292
TCCCGAGgtaagtg R: ggttctccacagggcagtgagcc
6 87 ccctcagGACATAC F: ccttgagcacatcccccagcc 430
CCTGCAGgtaaggc R: gaatcatagaggcaccgacctaagg
7 114 cttatagAACCTGC F: gctgagtgctctgaagtaggaac 329
CGCCAAGgtaacac R: ccctgtagaacctaacttggttc
8 144 tctgcagGATGGCG F: gctggcctcacagccataggtggc 399
CCTTCCGgtgggtg R: ctgaggctgggtgagtccagagc
9 79 ccccgagGGCTCGG F: cctactggtgagaaagaagcaggg 362
GTAAATGgtgagtc R: gctctggtcacctagagagtc
10 101 ttgacagGGAACGA F: gaagacttccctaggcaatgcagg 345
TGTACAGgtaccct R: catgtctggtgcaggtgctc
11 204 atcccagGTGACTG F: gccaggacacactcaaacggagg 390
CGACCAGgtgtgtg R: gcagacccacttagctttgggacc
12 153 tctctagGGAGCCA F: ccagggttaagggcacgtgaagac 414
CTTCAAGgtaggtg R: ggctggatctgtgagcaatctgc
13 165 ttcccagCTCCTGG F: ggcagctgcgaggaagatcg 383
TGTCACAgtgggtt R: ctgccactcaaactgcaatgacc
14 68 cttccagGCTGTGG F: cacctcacaggacagtaatgagg 450
CAGACCTgtaagta R: ccacagacctcctccaccctcagc
15 229 ctggcagCTTCCTG F: ggacactttggaggagctgcatgac 425
AATTGAGgtaagtt R: gcctcagtatctcttggagctgc
16 258 cgcacagGCCATAG F: gtaagacctacgtaaaccctggc 583
TGCTGAGgtgagta R: ggcacaggctgcctttgataacg
17 540 ctttcagACCCTCT F: gtgcctatcagattctcaaagggac 810
CTTCTAGtgtatgc R: gacagtggggattataccattgtgc
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Amplicon
Marker Primers (5'-3') (bp)
------- --------------------------- ----------
IVS9-CA F: cccagcctcacaggacctcagctg 293 to 315
R: gctcccacattccatactgtgtcc
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