Figure 3. Gene expression by reverse transcription-polymerase chain reaction

Gene specific polymerase chain reaction amplicons of reverse transcribed human RNA from ocular tissues and commercially available poly-A RNA tissue types. All amplicons were designed to span at least one intron-exon boundary with a size between 250 and 400 base pairs. Lane 1: Sclera. Lane 2: Cornea. Lane 3: Optic nerve. Lane 4: Retina. Lane 5: Lung. Lane 6: Skeletal muscle. Lane 7: Heart. Lane 8: Trachea. Lane 9: Kidney. Lane 10: Brain (from Ambion; all others from Clontech).