Figure 3. Effect of cell cycle synchronization on protein expression

Cell synchronization increases productive transfection efficiency of HTM cells Cells were transfected with plasmid DNA encoding GFP at 18, 19, or 20 h after synchronization. A: Microscopic fields from representative experiments comparing the effect of synchronization on GFP protein expression in two cell types. B: A graphical summary of GFP protein expression in HTM cells transfected 18-20 h after synchronization. C: The effects of transfection 19 h after synchronization on GFP protein expression in two cell types. Summaries were generated by counting digitally captured fluorescent images (at least 300 cells per condition). Statistical differences were assessed between synchronized and unsynchronized cells using Student's t-test (asterisks indicate a p<0.01).