Figure 3. IL-1β increases plasmin, uPA, and MMP-9 but not MMP2 relative to the control

Media from corneal organ cultures were collected at 0, 2, 5, and 24 h after the addition of IL-1β. Zymography detected the zymogen and active forms of MMP-2 and MMP-9 (aMMP-2 and aMMP-9) on gels containing gelatin (A), plasmin (Pln) and urinary plasminogen activator (uPA) on plasminogen (Plgn)-casein gels (B), and plasmin on casein gels (C). Although plasmin, uPA, and MMP 9 are increased, no specific increase was observed at 5 h.