Figure 4 of
Lin, Mol Vis 2005;
Figure 4. Effect of overexpression of SOD1 on gap junctions in intact lens
Lenses with or without overexpressing SOD1 were treated with 100 μM H2O2 for 24 h at 37 °C in the CO2 incubator. Whole lens gap junction activity was analyzed by dye transfer assay as described in Materials. Lucifer yellow and rhodamine dextran were injected into the superficial cortical fibers per injection site using a microinjection apparatus (Nanoliter 2000; World Precision Instruments, Inc., Sarasota, FL). Extent of dye transfer (diffusion distance of rhodamine-dextran subtracted from lucifer yellow diffusion distance in μm) as gap junction activity in the lens was determined by confocal microscopy. The results are shown in a bar graph; the error bars represent the standard error of the mean.