Figure 3 of
Lin, Mol Vis 2005;
Figure 3. Effect of overexpression of SOD1 on PKCγ activation by H2O2
The supernatants from whole lens extracts were used to immunoprecipitate endogenous PKCγ using PKCγ antisera. PKCγ enzyme activity was measured as described in Materials. PKCγ enzyme activity in the lenses before or after long-term (24 h) H2O2 treatments are shown. Transient activation of PKCγ by H2O2 (100 μM, 20 min) was also determined. Enzyme activity was expressed as percent of untreated specific PKCγ activity. Untreated specific PKCγ activity was expressed as 100%. Values for three independent experiments are summarized in a bar graph; the error bars represent the standard error of the mean.