Figure 2. Effect of overexpression of SOD1 on cataract formation induced by H₂O₂

Lenses with or without overexpressing SOD1 or EYFP were treated with 100 μM H₂O₂ for 24 h at 37 °C in the CO₂ incubator. The lenses were washed with phosphate-buffered saline (PBS) and were photographed to examine cataract formation. The representative lenses were shown in the top panel (A). Opaque lenses and clear lenses were counted and graphed (B). The experiments were carried out in triplicate.