Figure 5. Chaperone-like activity of zebrafish and human αB-crystallin at 37 °C

The target proteins used were 0.6 mg/ml α-lactalbumin (A), 0.4 mg/ml insulin (B) and 0.1 mg/ml lysozyme (C). Curves show the aggregation of α-lactalbumin (Lac), insulin (Ins), or lysozyme (Lys) alone or with varying amounts of zebrafish αB-crystallin (ZaB) or human αB-crystallin (HaB). Shown are the mass ratios of α-crystallin to target protein in each assay. Lower absorbance indicates greater protection from aggregation provided by the crystallins. The decrease in absorbance over time in the 1:1 zebrafish αB-crystallin:lysozyme reaction is due to precipitation of the aggregated protein. Reaction buffer conditions are described in Methods.