Figure 6 of
Ohno-Matsui, Mol Vis 2005;
Figure 6. Increased secretion of thrombospondin-1 in RPE cells cultured on amniotic membrane
Concentration of immunoreactive thrombospondin-1 in supernatants of RPE cells cultured on either plastic or amniotic membrane. Cells were incubated in serum free medium for 48 h before harvest for protein concentration, and the amount of secreted thrombospondin-1 in RPE-conditioned culture medium was determined using commercial ELISA kits. The data show that thrombospondin-1 secretion is increased in RPE cells cultured on amniotic membrane compared to cells on plastic (p<0.01).