Figure 5 of
Ohno-Matsui, Mol Vis 2005;
Figure 5. Increased secretion of VEGF in RPE cells cultured on amniotic membrane
Concentration of immunoreactive VEGF in supernatants of RPE cells cultured on either plastic or amniotic membrane. Cells were incubated in serum free medium for 48 h before harvest for protein concentration, and the amount of secreted VEGF in RPE conditioned culture medium was determined using commercial ELISA kits. The data show that VEGF secretion is increased in RPE cells cultured on amniotic membrane compared to cells on plastic (p<0.01).