Table 2 of Usui, Mol Vis 2004; 10:832-836.

Table 2. Genes upregulated or downregulated on corneal NV

To identify the genes that are expressed in the process of corneal neovascularization, we compared phosphorescent images of the arrays hybridized with 32P labeled cDNA probes generated from RNA preparations of normal and vascularized corneas. Intensity differences greater than 3 arbitary units between normal and vascularized corneas were accepted as significant and selected for further study. Among the 545 immune related genes tested, 6 genes were upregulated and 1 gene was downregulated in the vascularized corneas compared with normal cornea. In the vascularized corneas, the expression of calreticulin (calregulin), HMGI(Y), apolipoprotein E (apo E), HSP84 (HSP90β), pleiotrophin, and IGFBP-5 were upregulated. In contrast, interferon regulatory factor-1 (IRF-1) was decreased in vascularized corneas compared with normal corneas.

                                             Intensity    Accession
                     Gene                    difference    number
    --------------------------------------   ----------   ---------


    Calregulin (calreticulin)                  -7.8        M92988
    HSP90β (HSP84)                             -6.17       M36829
    HMGI(Y)                                    -4.09       J04179
    PTN (pleiotrophin)                         -3.71       S52357
    IGFBP5                                     -3.69       X81583
    Apo-E(Apolipoprotein E)                    -3.49       M12414
    GRP94 (Endoplasmin, GP96)                  -2.55       J03297
    IGFBP6                                     -2.41       X81584
    patched                                    -2.24       U46155


    Interferon regulatory factor-1 (IRF-1)      5.84       M21065
    HSP70                                       1.95       D85732
    Adenosine A2A-R                             1.62       Y13346

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©2004 Molecular Vision <>
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