Table 1 of
Fujii, Mol Vis 2004;
10:814-820.Table 1 of
Fujii, Mol Vis 2004;
10:814-820.
Table 1. Oxidation and racemization of αA-crystallin
This table indicates that after UV-C irradiation, the relative abundance of Met1-oxidized T1 peptide in α-crystallin increased, reaching an abundance five times higher than that of native T1 peptide at a UV-C dose of 5 J/cm2. With higher doses of radiation the abundance of peptide decreased. We have reported previously that Asp151 in αA-crystallin is stereochemically labile to allow the conversion of L-Asp to D-Asp during UV-B irradiation. As shown in column 3, the D/L ratio of Asp151 doubled with a UV-C irradiation dose of 1 J/cm2 and then decreased gradually with increasing doses of radiation.
D/L of
Irradiation (T1+16)/T1 Asp151
----------- ---------- ------
0 0.27 0.13
1 0.48 0.27
2 1.63 0.24
5 1.4 0.22
10 1.18 0.2
20 0.55 0.15
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