Figure 4. Bis ANS fluorescence spectra of normal and UV-C irradiated α-crystallin

The change of hydrophobicity of α-crystallin by UV-C irradiation was measured by fluorescence spectra after the Bis-ANS assay, as described in Methods. The excitation wavelength was 395 nm. The protein concentration was 0.1 mg/ml in 50 mM Tris HCl buffer (pH 7.8).