Table 1 of
Cassar, Mol Vis 2004;
10:808-813.
Table 1. Primers used for the RT-PCR expression analysis
These primers anneal to regions of the cDNA that flank sequences between exons 3 and 4 (Set A) or between exons 4 and 7 (Set B). Their names indicate the region of the cDNA to which they anneal and the direction of their extension by polymerase.
Primer Set Name Sequence --- ------- ----------------------------------------------- A Exon 3F 5'-AGC CCA CTC GTC AAG TGA GGA GAC ATC GTC A-3' Exon 4R 5'-TGG TGC ACA TGC CTG GCA GCC CAC CTG CGC A-3' B Exon 4F 5'-TTC CGT GGA GCC GAG CTT AAG GAG GTG TCC A-3' Exon 7R 5'-AGG TTG CTG GAC GGG TCC ACC ACG ATC GCA T-3' |