Figure 5 of
Yin, Mol Vis 2004;
Figure 5. M1 receptor gene promoter exists in rat but not chick genome
PCR, Southern and subsequent sequencing analyses of chick and rat genomic DNA suggested the M1 receptor promoter region was confined to the rat alone. PCR amplification with M1 promoter primers at an annealing temperature of 60 °C. Four different MgCl2 concentrations, 1.5 mM, 2.5 mM, 4 mM, and 5 mM MgCl2, were used. DNA fragments of the expected size (212 bp) were observed in rat positive controls (R). Fragments amplified from chick (C) genomic DNA were sequenced and found to be related to non-coding chromosomal regions. Results of Southern analysis (shown on the right) further confirmed the PCR results.