Figure 4 of
Yin, Mol Vis 2004;
Figure 4. M1 receptor gene exists in rat but not chick genome
PCR, Southern, and subsequent sequencing analyses of chick and rat genomic DNA suggested the M1 receptor gene was confined to the rat alone. PCR amplification of genomic DNA with primer M1a (A) and M1b (B). An annealing temperature of 58°C and four different MgCl2 concentrations, 1.5 mM, 2.5 mM, 4 mM, and 5 mM MgCl2, were used. In all rat positive controls (R), DNA fragments of the expected size (207 and 385 bp with primers M1a and M1b, respectively), were observed. Fragments amplified from chick (C) genomic DNA (arrows) were sequenced and found to be related to non-coding regions of chromosomes. PCR findings were confirmed by Southern analysis (shown on the right).