Figure 7 of
Liang, Mol Vis 2004;
Figure 7. Gene structure for LOC313850
A: Rx-3132-51 is located within hypothetical gene LOC313850, which encodes a PLCXc containing domain. The top line depicts the gene structure with exons 1-4 as gray boxes and cryptic exons as dashed boxes. The location of Rx-3132-51 is shown as a thick line underneath the gene structure. Forward and reverse PCR primers (p1 and p4) are shown as arrows. Asterisks indicate the exons containing the PLCXc domain. A schematic of the putative translation product is shown below the gene structure with the PLCXc domain positioned between amino acids 84-215. B: The PLCXc domain from phosphatidylinositol dependent phospholipase C (PI-PLCXc) is compared with with the PLCXc domain from LOC313850. Matches are shown between the two sequences. Matches to PI-PLCXc residues are shown in lower case letters. Similar amino acids are marked with a plus sign (+).
Consensus(PI-PLC-X) DMSIPLSHYFISSSHNTYLTGKQLWGKSSVESYRQQLDAGCRCVELD is+ ++ + w+ ++++ +r ql+ag+r+++l+ LOC313850 84 ----------ISLVKKL----MKKWSVTQNLTFREQLEAGIRYFDLR Consensus(PI-PLC-X) CW....DGKPDDEPIIYHGHTLTLEIKLKDVLEAIKDFAFKPTSPYPVIL + ++++d+ d+e hg ik d l +i +f++ + + +i LOC313850 117 VSskpgDT--DQEIYFIHG---LFGIKVWDGLMEIDAFLTQ-HPQE-IIF Consensus(PI-PLC-X) SLENHCNSDDQQRKMAKYFK..EIFGDMLLTKPTLDSLTTEPGLPLPSLK +++nh++ +d++++ ++ +e fg +l p + e ++ l sl+ LOC313850 160 LDFNHFYAMDESHHKCLVLRIQEAFGNKLC--P---ACSVE-SMTLRSLW Consensus(PI-PLC-X) DLRGKILLKNKK + ++ l++ ++ LOC313850 204 EKKYQVLIFYHC 215