Figure 7 of Liang, Mol Vis 2004; 10:773-786.


Figure 7. Gene structure for LOC313850

A: Rx-3132-51 is located within hypothetical gene LOC313850, which encodes a PLCXc containing domain. The top line depicts the gene structure with exons 1-4 as gray boxes and cryptic exons as dashed boxes. The location of Rx-3132-51 is shown as a thick line underneath the gene structure. Forward and reverse PCR primers (p1 and p4) are shown as arrows. Asterisks indicate the exons containing the PLCXc domain. A schematic of the putative translation product is shown below the gene structure with the PLCXc domain positioned between amino acids 84-215. B: The PLCXc domain from phosphatidylinositol dependent phospholipase C (PI-PLCXc) is compared with with the PLCXc domain from LOC313850. Matches are shown between the two sequences. Matches to PI-PLCXc residues are shown in lower case letters. Similar amino acids are marked with a plus sign (+).

A:

(6 K)

 

B:


  Consensus(PI-PLC-X)   DMSIPLSHYFISSSHNTYLTGKQLWGKSSVESYRQQLDAGCRCVELD
                                  is+  ++     + w+ ++++ +r ql+ag+r+++l+
  LOC313850    84       ----------ISLVKKL----MKKWSVTQNLTFREQLEAGIRYFDLR

  Consensus(PI-PLC-X)   CW....DGKPDDEPIIYHGHTLTLEIKLKDVLEAIKDFAFKPTSPYPVIL
                        + ++++d+  d+e    hg      ik  d l +i +f++   + + +i
  LOC313850   117       VSskpgDT--DQEIYFIHG---LFGIKVWDGLMEIDAFLTQ-HPQE-IIF

  Consensus(PI-PLC-X)   SLENHCNSDDQQRKMAKYFK..EIFGDMLLTKPTLDSLTTEPGLPLPSLK
                        +++nh++ +d++++    ++ +e fg +l   p     + e ++ l sl+
  LOC313850   160       LDFNHFYAMDESHHKCLVLRIQEAFGNKLC--P---ACSVE-SMTLRSLW

  Consensus(PI-PLC-X)   DLRGKILLKNKK
                        + ++  l++ ++
  LOC313850   204       EKKYQVLIFYHC   215

Liang, Mol Vis 2004; 10:773-786 <http://www.molvis.org/molvis/v10/a92/>
©2004 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535