Table 3 of
Tasheva, Mol Vis 2004;
10:758-772.Table 3 of
Tasheva, Mol Vis 2004;
10:758-772.
Table 3. Gene specific primers used for human and bovine SLRPs
Primer pairs used for amplification of indicated human and bovine SLRPs and their corresponding amplicons are shown. The primer pair for human mimecan detects two differentially spliced mRNA isoforms. The first letter of the primer name indicates whether the primer is human (H) or bovine (B).
Accession Primer Amplification
Gene number name Primer sequence (5'-3') product (bp)
-------------- --------- --------- ---------------------------------------------- -------------
Mimecan NM_033014 Hmim+1 GGCTAATGCACAGACATGAACATCTATTGAGG 506 & 230
Hmim-506 GCGTGAGTCCTGCTGGGTTGGTGG
Decorin NM_001920 Hdec+124 GGGCTGGACCGTTTCAACAGAGAGG 586
Hdec-710 GTATCAGCAATGCAAATGTAGGAG
Biglycan NM_001711 Hbgn+964 GATCAGGATGATCGAGAACGGGAG 280
Hbng-1244 GGTCAGTGACGCAGCGGAAAGTGG
Mimecan M37974 Bm+411 TTTAGGATCCATTAAAATGAAGACTCTGCAATCTACACTTCTCCTG 902
Bm-1313 GATAGCTCGAGAATGTATGACCCTATAGG
Keratocan U48360 Bkera+150 ATGGATCCATGGCATCCACAATCTGCTTCATCCTCTGGG 189
Bkera-339 CAGTACAAAGCAGTAGGGAAACTGGGGG
Lumican L11063 Blum+671 TGACTTGAGCTTCAATCAGATGACC 503
Blum-1174 CCATAAACTGCTGTTCCAGGCTACACC
Chondroadherin NM_174019 Bchad+595 CTCAGTTCCCTGCAGCCCGGCGCTC 272
Bchad-867 CATGTTTCAGCGTGGTCACACCC
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