Figure 7. Semi-quantitative RT-PCR analysis of SLRPs expression in bovine corneal keratocytes

Semi-quantitative RT-PCR was performed to assess the effect of Runx2 isoforms on SLRP expression in bovine corneal keratocytes. A: Photographs of ethidium bromide stained agarose gels. The 902 bp transcript corresponding to mimecan, the 189 bp transcript corresponding to keratocan, the 503 bp transcript corresponding to lumican, and 272 bp transcript corresponding to chondroadherin were amplified together with QuantumRNA 18S internal standard in the same (multiplex) PCR reaction. B: Plot of the mean values of relative quantities of RNA obtained from two experiments.