Figure 6. Semi-quantitative RT-PCR analysis of SLRPs expression in MG-63 cells

MG-63 cells were transiently transfected with the indicated Runx2 expression vectors. Total RNA was isolated and semi-quantitative RT-PCR was performed to assess the effect of Runx2 isoforms on SLRP expression. A: Photographs of ethidium bromide stained agarose gels. The 230 bp differentially spliced transcript corresponding to OGN/mimecan, the 586 bp transcript corresponding to decorin, and the 280 bp transcript corresponding to biglycan were amplified together with QuantumRNA 18S internal standard in the same (multiplex) PCR reaction. To modulate amplification efficiency of 18S rRNA, 18S primers were mixed with 18S competimers in a 3:7 ratio. B: Plot of the mean values of relative quantities of RNA obtained from two experiments.