Table 2 of
White, Mol Vis 2004;
10:738-749.Table 2 of
White, Mol Vis 2004;
10:738-749.
Table 2. PCR and sequencing primers
Primers were designed based on sequence provided by accession numbers (indicated in parenthesis) through a search of the GenBank database. Gene Runner software version 3.0 (Hastings Software, Inc., Hastings, NY) was used to design primers.
Nucleotide
Product amplified Primer name Sequence (5'to 3') Position
------------------ --------------------- ------------------------------------ ----------
PDE2 (U67733) JT5 (RT-PCR) CCCAAAGTGGAGACTGTCTACACCTAC 387- 413
JT6 (RT-PCR) CTGGCCACAGTGCACCAAGATGA 643- 665
PDE5 (XM_003559) JT7 (RT-PCR) GTGAAAGATATTTCTAGTCACTTG 786- 805
JT8 (RT-PCR) ATACATGTAATTGATTCTGTTTGC 1530-1553
PDE7A (U67932) JT9 (RT-PCR) GCTCTCTTCGGCTGCCCCAAT 150- 170
JT10 (RT-PCR) ACGAAGTTTCATCATATCTAA 615- 635
PDE4 (U97584) JT69 (RT-PCR) ATGGT(TAG)GA(AG)AC(GC)AA(GA)AA(GA)GT 979- 998
JT65 (RT-PCR) AG(GA)TC(GCT)GCCCA(GAT)GT(GCT)TCCCA 1264-1283
PDE1A (U40370) PDE1A-F (RT-PCR) GGTGGCCCAGTCACAAATAGG 1320-1340
PDE1A-R (RT-PCR) TGAGCCCGGTGAATTAAGGAG 1861-1881
PDE1B (U40584) PDE1B-F (RT-PCR) TGGCCCAGGTCTTCATTGAGTC 1734-1755
PDE1B-R (RT-PCR) CCCAGACCTGCCAGCTAAGATC 2156-2177
PDE1C (U40371) PDE1C-F (RT-PCR) TCAATGGAACACGGGCAAAC 1293-1312
PDE1C-R (RT-PCR) TTGGAGGTGTGTCCTGCTGTG 1761-1781
PDE6α (M26061) HA-1 (sequencing) GGACGTTAGCAATCTTCTT 562- 580
HA-2 (sequencing) GACTTTGTGGACATCCTCACAG 610- 631
HA-3 (sequencing) TTCACAGTTGTGCAGGTAACT 802- 822
HA-23 (sequencing) TGTGGTCTGGGAGCAAAGTC 845- 864
HA-D5 (sequencing) TGATGTGTGGCCGGTTCTGATG 987-1008
HA-D4 (sequencing) CCGGAGTCCTGGGACCAGAGTAA 1023-1045
HA-4 (sequencing) CTTTTACAAGGTCATTGACTA 1062-1082
PDA6α HA-5 (sequencing) GACTTTGATGTCCTCTTTGCCAT 1091-1113
HA-A6 (sequencing) CAGGCCATTCTGGGCAACATAAGT 1162-1185
HA-6 (sequencing) AACATCATGAACGCGCCTTCAGA 1192-1214
HA-8 (sequencing) CCTGACACCTATGAGTCAATGAA 1420-1442
HA-9 (sequencing) TTCTTCATTGTCACACTTCAC 1492-1512
HA-10 (sequencing) AGAGAGGTGTATGGGAAGGAGCC 1534-1556
PDA6α HA-A10 (sequencing) AGAGGAGCTGGCTGAGATCCTG 1572-1593
HA-12 (sequencing) GAGCTGCCAGATGCAGATAAAT 1600-1621
HA-13 (sequencing) GTGAAATTTATCCACCACTTTGAGC 1704-1728
HA-D13 (sequencing) GGCTACCGCAAGATCACCTACC 1774-1795
HA-14 (sequencing) GCTGAAGCGCTACTTCACGGACC 1857-1879
HA-15 (sequencing) GACCACAGAGGCACCAATAACC 1924-1945
HA-21 (sequencing) GTAGACGAAGGTGCAAACAAAG 2454-2478
HA-16 (sequencing) ACGATGTTCCAAAAGATCGTGG 2155-2176
HA-18 (sequencing) GATGATGACCGCCTGTGATCT 2262-2282
W57 (sequencing) AACAATCGCAAGGAGTGGAA 2530-2549
HABW-R (sequencing) GAAGATGCATTTCTCTGTC 339- 359
HA-F (PTT) GTAGATCTCCACCATGGGCGAGGTGACAGCAGA 121- 140
HA-R (PTT) GAAGATCTTTACTGGATGCAGCAGGACTTG 2682-2703
PDE6β (S41458) HB-F (PTT) CAGGATCCACCATGAGCCTCAGTGAGGA 22- 38
HB-R (PTT) CAGGATCCTCACAGGATACAGCAGGTTGAA 2565-2586
PDE6γ (M36476) HG-F (PTT) CAGGATCCACCATGAACCTGGAACCGCCC 102- 119
HG-R (PTT) CAGGATCCCTAGATGATGCCATATTGGGC 345- 365
PDE6δ (AF045999) HD-F (PTT) GTAGATCTCCACCATGTCAGCCAAGGACGAGCGGG 150- 171
HD-R (PTT) GAAGATCTTCAAACATAGAAAAGTCTCACT 581- 602
PDE6β (S41458) BetaNheI-F (promoter) TGGGCTAGCACCATGAGCCTCAGTGAGGA 19- 38
BetaNheI-R (promoter) TGGGCTAGCTCACAGGATACAGCAGGTTGAA 2583-2564
PDE6β (S41458) HB2-F (sequencing) ATATGCTGGCCACACCCATC 542- 561
HB3-R (sequencing) CGCCAGTTGTGGTAGGTGATTC 1679-1700
HB4-R (sequencing) GCATGGACAGCACATTCTTG 1161-1180
HB5-F (sequencing) AGGTCATTCCCACACCCTCAG 1004-1024
PDE6B-F (sequencing) GTGCTGCTGTGGTCGGCCAAC 733- 753
PDE6B-R (sequencing) CCGGCCATCAGGCGTGCGTGG 926- 948
PDE6δ (AF045999) Delta-F (RTPCR) CGATCATGTCAGCCAAGGACG 154- 174
Delta-R (RTPCR) GCCTCTATCAAGGACTGCCAG 2149-2169
PDE6γ (NM_002602) RodGamma-F (RTPCR) GCCTCACCATGAACCTGGAAC 96- 116
RodGamma-R (RTPCR) CGTGCTAGATGATGCCATATTG 350- 371
PDE6γ' (NM_006205) ConeGamma-F (RTPCR) CTGAAAGGGAAACATCAGCC 48- 67
ConeGamma2-F (RTPCR) Gacaacactactctgcctgctc 91- 112
ConeGamma-R (RTPCR) CTTCAGATAATCCCAAACTGAG 317- 338
PDE6α' (XM_005906) ConeAlpa-F (RTPCR) ACAAGGAGATCTGGAGAGAAC 2242-2262
ConeAlpha-R (RTPCR) TTTCTTCATAGTCACAGGGTAG 2848-2869
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