Table 1 of
Nakamura, Mol Vis 2004;
10:703-711.
Table 1. Target and duplex sequences for TβRII specific siRNAs
Four siRNAs, designated as hT1, hT2, hT3, and hT4, were designed from the coding sequence of the human TβRII gene. The target sequences (5'-3') and the siRNA duplex sequences are listed, with the position of the first nucleotide in the human TβRII sequence shown in parentheses. siRNA from the similar region as hT1 of the mouse TβRII sequence was also designed and the target sequence of the mouse T1 (mT1) siRNA is shown. There is one nucleotide difference (small letter) between the human and mouse TβRII sequences.
siRNA
duplex Species TβRII sequence (5'-3') siRNA duplex sequences
------ ------- --------------------------- -------------------------
hT1 Human (529)AATCCTGCATGAGCAACTGCA 5'UCCUGCAUGAGCAACUGCAdTdT
dTdTAGGACGUACUCGUUGACGU
hT2 Human (1253)AGCATGAGAACATACTCCAG 5'GCAUGAGAACAUACUCCAGdTdT
dTdTCGUACUCUUGUAUGAGGUC
hT3 Human (1113)AAGGCCAAGCTGAAGCAGAAC 5'GGCCAAGCUGAAGCAGAACdTdT
dTdTCCGGUUCGACUUCGUCUUG
hT4 Human (948)AAGACGCGGAAGCTCATGGAG 5'GACGCGGAAGCUCAUGGAGdTdT
dTdTCUGCGCCUUCGAGUACCUC
mT1 Mouse (195)AgTCCTGCATGAGCAACTGCA 5'UCCUGCAUGAGCAACUGCAdTdT
dTdCAGGACGUACUCGUUGACGU
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