Figure 1. Primer and probe design

The region of the HEMICENTIN-1 gene containing the A16,263G variant was amplified with the primers FIBL-6 F mut and FIBL-6 A (blue arrows). The position of an A in the FIBL-6 F mut that was introduced to disrupt a predicted stem-loop structure (shaded boxes) is indicated with a solid triangle. The 3' fluorescein labeled anchor probe (FIBL-6 FL) is indicated by an orange arrow and the 5' LC Red 640 labeled sensor probe (Sensor C) by a green arrow. The sensor probe spans the site of the A/G variation (indicated by a solid triangle). Pink arrows indicate a predicted stem-loop.