Figure 5. The time course of light to dark translocation of arrestin and α-T

A: Temporal kinetics of light to dark translocation of arrestin and rod α-T are shown. The Arrestin graph in red indicates ROS/RIS as a percentage of ROS/RIS at 60 min versus the specified time. The Transducin graph in green indicates ROS/RIS as a percentage of ROS/RIS at 0 min versus the specified time. Each point in the time course experiments is from a single animal and represents the average values (the error bars represent the stardard deviation) from three different fields within a section. These data are representative of those obtained from three separate experiments. B: The same data are shown on an expanded time scale. Densitometric scanning of grayscale images and calculation of ROS/RIS ratios confirm that maximal compartmentalization of arrestin occurs within 18 min of dark adaptation whereas α-T requires more than 200 min.