Figure 3 of
Fernandes, Mol Vis 2004;
Figure 3. Effect of high glucose on expression of GLUT1 in TR-iBRB cells
A: In TR-iBRB cells exposed to high glucose concentration there is a subnormal expression of GLUT1 protein. The cells were solubilized in SDS-PAGE buffer and equal amounts of protein (30 μg) were loaded on a 12% polyacrylamide gel. The proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with an antiserum against GLUT1. Bars represent standard errors (3 rats/group). The asterisk (*) indicates p<0.05. B: There are no significant changes in the levels of GLUT1 mRNA after 48 h of hyperglycemia. The total RNA was isolated from control and high glucose treated TR-iBRB cells and northern blot analysis was performed. Bars represent standard errors (3 rats/group).