Figure 2 of
Fernandes, Mol Vis 2004;
Figure 2. Effect of high glucose on GLUT1 expression in primary cultures of BREC cells
In BREC cells exposed to high glucose concentration there is a decrease in the amount of GLUT1 protein. The cells were solubilized in Laemmli buffer without β-Mercaptoethanol and equal amounts of protein (30 μg) were loaded on a 12% polyacrylamide gel following addition of reducing agent β-Mercaptoethanol. The proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with an antiserum against GLUT1. Coomassie staining is used as a control for total protein loading.