Table 1 of
de Iongh, Mol Vis 2004;
10:566-576.Table 1 of
de Iongh, Mol Vis 2004;
10:566-576.
Table 1. PCR primers
Identification of receptors expressed in postnatal rat lenses by RT-PCR. Primers are listed 5' to 3' along with the size of the fragment amplified. Expression of BMP and activin receptors in RNA isolated from P3 and P21 rat lenses is indicated qualitatively.
TGFβ Expected Expression
family fragment in P3/P21
receptor Primers (5'-3') size lens (RT-PCR)
-------- -------------------------- -------- -------------
BMPR II AGGGATGACTTCCTCGCTGC 687 bp +++
AACTGGACGCTCATCCAAGG
Alk3 ATGACTCAGCTATACACTTACATCAG 629 bp +++
(BMPRIA) CAGGTCTTTCAGTGATTCTCC
Alk6 ATGCTCTTGCGAAGCGCTGG 493 bp -
(BMPRIB) TCCAGCCCAATGCTGTACCG
ActRIIA ATGGGAACTGCTGCAAAGTTG 872 bp ++
ACAAAGTTCATTCCAAGAGACC
ActRIIB GTATTTCTGCTGTGAAGG 455 bp ++
GACTGCTTGTCCTGAAGTGG
Alk1 TTGGGGAGCTTCAGAAGGG 500 bp -
CTGTTCAGATGCCTTCAGG
Alk2 TTATACAATGGTCGATGGAGC 482 bp -
(ActRIA) TTCAGGCGCTCTTGATTGC
Alk7 CTCAAACTTTACCTGCCAAACC 515 bp -
AACGACCTTTTCCTACGATTT
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