Figure 2. Sprouting from HREC aggregates

Sprouting from HREC aggregates at 3 days, controlled by pro- and anti-angiogenic factors. A: HREC aggregate maintained in collagen in non-supplemented culture medium. B: HREC aggregate outgrowth, stimulated with VEGF and bFGF (both at 2.5 ng/ml). C: Inhibition by 10 mm endostatin of the sprouting from HREC aggregates cultivated in presence of VEGF and bFGF. Phase contrast micrographs. Original magnifications: x 80.