Figure 6. Immunohistochemical localization of Chad in selected tissues represented on the ZE1 tissues microarray slide

Immunoreactivity for Chad was found in Purkinje cells of the cerebellum and several peripheral nerves, including peripheral nerves in the orbit, the tongue, and skeletal muscle. In the heart, immunoreactivity for Chad was associated with blood vessels. In the gastrointestinal tract, Chad expression was localized to enteroendocrine cells of the stomach and intestine. Strong expression was also observed in Meissner's nerve plexus of the stomach, intestine, and colon. Intense immunoreactivity for Chad was also found in the cells of the pancreatic islet and follicular cells in ovary. Positive immunostaining appears brown and is highlighted by arrows.