Figure 1. Semi-quantitative RT-PCR performed to assess Chad expression in mouse and human tissues

A: Schematic, showing part of the human Chad gene and primers for PCR amplification of Chad cDNA. The primers were designed to flank an intron and to amplify a 280 bp cDNA product. The genomic structure of mouse Chad is similar to that of human; mouse primers also amplify a 280 bp cDNA product. B: Chad expression in mouse tissues. The 280 bp PCR product corresponding to Chad cDNA was amplified together with QuantumRNA 18S internal standard in the same (multiplex) PCR reaction. To modulate amplification efficiency of 18S rRNA, 18S primers were mixed with 18S competimers in a 3:7 ratio. PCRs shown are representative of at least two repetitions of each PCR. C: Chad expression in human tissues. The 280 bp PCR product corresponding to Chad cDNA was amplified together with QuantumRNA 18S internal standard in the same (multiplex) PCR reaction. To modulate amplification efficiency of 18S rRNA, 18S primers were mixed with 18S competimers in a 3:7 ratio. PCRs shown are representative of at least two repetitions of each PCR. The "M" labels a lane with a Hi-Lo DNA molecular weight marker.