Figure 6. Effect of variable oxygen on PEDF

A: RT-PCR analysis of PEDF mRNA from whole homogenized retina in RA control and in 50/10 OIR model. P7 RA control (lane 1); P7 50/10 OIR model that had 3.5 cycles of 24 h of 50% oxygen followed by 24 h of 10% oxygen (lane 2); P14 RA control (lane 3); P14 50/10 OIR model that had 7 cycles of 24 h of 50% oxygen followed by 24 h of 10% oxygen (lane 4); P18 RA control (lane 5); P18 50/10 OIR model that had 7 cycles of 24 h of 50% oxygen followed by 24 h of 10% oxygen followed by 4 days in room air (lane 6). PCR was repeated four times on different experiments and this result is representative. B: Densitometry measurements using 18S RNA as the control gene. Samples were assayed in triplicate and error bars are standard deviations.