Table 1 of
Mansergh, Mol Vis 2004;
10:490-511.Table 1 of
Mansergh, Mol Vis 2004;
10:490-511.
Table 1. Significant genes: Nine month Sparctm1cam129Sv/Ev lenses versus 129Sv/Ev controls
Significant genes included in this table are those giving a significance level of delta=0.5 using SAM and that have a fold change of more than two in 8/10 repetitions. All significant genes are listed here, including those that were not confirmed by PCR. The columns list the GenBank accession number and primer name, and the forward and reverse primer sequences. The primer name is not listed where the EST is uncharacterized, where ESTs correspond to known genes, the gene name is used. Primer sequences for housekeeping genes are listed at the bottom; these are standard house keeping gene primers used by us before and are not directly associated with any ESTs on the array, therefore no accession numbers are given.
Accession Primer number Name Foward primer Reverse primer ---------- -------- --------------------------- ------------------------- BG079989 Alb1 CTACACCCAGAAAGCACCTCA CTCAGCAAGAGCCGTTTGTTT AW546550 CAGGGACAAAGGAAAGGCATAG CTGCTTGCTGGTGATAGTTGG AW546837 AACATACTTCAACTGTAGGCAAAATAG TGGAGTTTCTGGAGGATAATAAGG AW549180 GCTCGCTGGTAGCTCTTTGG CTGGTGCCTTGTCTGCTTTG AW550210 CTGTATTGATTGAGCCACCAACT CGTCTGAGGGAAACTGGGTG AW550998 GTGGACTACAGCCTCAAACTGG GGAGACAGAGCAGGGATGGTT BG065042 GCCCTGAAGTAAGAACCAGATGTC CGCCACCAGCACCCAAAG BG072975 GCACAAGAGGGAAATAAAGGTG GTGAACCCACATCCACGAGTT BG073080 TAGCTCTTGCCCTGTGGTTC CCATACCATTTGCTGCTTTTCTC BG075008 TTCATGGCAGCTGGTCTGC GCATGGCTTGGTGTATCTG BG076590 TAGAGCAAGTTCGGATTCAGGTT GAGCGGGCGGATAAACACT BI076713 TTGACTGATTGTGTGGGCTTG CGTGAGGAACTGAGGGTTGAG BG077309 Birc5 TGAATCCTGCGTTTGAGTCGT GTTCCCAGCCTTCCAATTCCT C79876 C79876 AGAGATGGGTCCTCACAAGCA GGTCAAAGGCTGGCACTGG C80273 C80273 TTTATGGCAGGGTTAGGCTGGG CACACACCGAGGCTACTCCA C75991 Cbx5 GGTTGGTTGCTTTGGGAGATT TGTTATGGGAGGGTATGTGAGG BG073735 Col1a2 TGCTGAATCTAATGAAGAGAGAACC TGACCAAGGCTGACACGAAC BG065049 Col2a1 GTTCAGCCCCTCCAATGTCC CTGTAAGAACAGCATCGCCTACC BG074327 Col3a1 AAGGCTGAAGGAAACAGCAAA TTGGTCACTTGCACTGGTTGA BG085518 EraF TGATCTCCACAGGGATAAAGGAG AGGAGGGCAGTGTATTGCTTG BG087506 Glud ATCCAATGCACCCAGAGTCAA TTCCACCATGCTTTCCAAACT BG071068 Gnb1 GTGGCAAAGAGGCAGCAGAG CACACATATCGGAAGCAGCAAG BG078478 Gpc3 CAGGTCCGTTCTTTCTTCCAG TTGAACATGGCGTTGGTGTAG BG065213 Grb10 TGGTTACACGGAGTCGTCATTTAG CCTTGCCGAAGTGAATTGTTG BG076718, H19 GTATGCCCTAACCGCTCAGTC GCTCTTCGAGACACCGATCAC BG086671, BG087028, BG087529, BG087637, BG087638 BG065110, H2afz TACCATTGCTGGTGGTGGTGT TTTGGTTGGTTGGAAGGCTAAT BG076995 BG079788, Hba-a1 CCACCACCAAGACCTACTTTCC AGGCAAGGAATTTGTCCAGAGA AW550167, AW551388, BG072574, BG072760, BG073467, BG073468, BG073469, BG073608, BG073617, BG074904, BG085818, BG086202, BG086243, BG086422 Hba-X GCGGTTAAGAGCATCGACAAC TTCTCAGTCAGGATAGAAGACAGGA Hbb-b1 ACCTGACTGATGCTGAGAAGG CCCTTGAGGCTGTCCAAGTG AW548291, Hbb-b2 CACCTGACTGATGCTGAGAAGT CCCTTGAGGTTGTCCAGGTTT AW548342, BG072990, BG085433 Hbb-Y TTACTGCTGAGGAAAAGACCCTC AAGTGACTAGCCAAAACAATCA HbbZ TACCTCACACCATGGTTCAC GTACTTGTGGGACAGAGCAT BG085427 Hmgb2 GCGAGGAGCACAAGAAGAAG TCAGAGCAAAACAGGAAGAAGG BG064815, Igf2 TGGAACATTGGACAGAAACTCA GACAGTAGGGAAGAGACAAGATGGA BG073613 BG066442 Kpna2 CCCTTTCTTGTTGGTGTCCTC ATCCAAGCCTCCACACTCTTC BG076937 Lmnb1 GACATGGAGATCAGCGCCTAC TCAAGCGAATAAACTTCCCATC BG085378 Marcks TCAGATAGTTGTTTCCACCATTCC TTCCTTTTGACCCACCCATC BG087241 est CTCAAAGACGGAGGTGTGCTT TGTGGGTAGTGGCTAATGTGG BG074746 Mscp TGGAGGGTGATGGTTGTGAG CTCGGTCATTGGCATCTTTG BG063925 Mt2 CGTGGGCTGTGCGAAGTG GTGGAGAACGAGTCAGGGTTG AW553287 Osf2 GTTTTCTGAATTACCAACTCTG CACAGTTACCTTTCCAGGGG BG086397 P4hb AGCTCTGGCTCCCGAGTATG GATGACCGTCACTTCGCTTG BG070102 Phtf GCCGAAGCTCCATTCGTTAG AAGGAGCTGGACACACCATTT BG074984, Sfpq ATGGGTGGTGGTGGAACAA CCCTTCATACTCTTCTCTCCCTCT BG086821 BG064802, Sparc GCTGTGTTGGAAACGGAGTTG CTTGCCATGTGGGTTCTGACT BG072874 AW548440 Syn1 CACAGTCCACAAAGGCTTTTTATT CCTCGGCTGGCATTATGGAG BG086002 Tia1 CGTTTCGTGAACTATACCATCTCC GGAATCCTTGAGGCACCTTCT BG086372 Tmpo CTTCACAGCATTTTCGTATAGATGG TGGTTTCTTTGGTGTTCTTCTGG Housekeeping genes GAPDH Gapdh ACCACAGTCCATGCCATCAC TCCACCACCCTGTTGCTGTA β-Actin β-actin CGTGGGCCGCCCTAGGCACCA TTGGCCTTAGGGTTCAGGGGG Crystallin CryβA1 GCTCCTGCCCAAATGTCTCTG GATGGGTCGGAAGGACATGAG βA1 |