Figure 2. Localization of ANP-Immunoreactivity in epiretinal membranes

A Hematoxylin and eosin stain. Scale bar represents 100 μm. B Positive staining for ANP was observed in both vascular endothelial cells (EC; white triangles) and vascular smooth muscle cells (VSMC; white stars) D: ANP staining shown by fibroblast-like cells (arrows). C, E Positive immunostaining was abolished when the adjacent sections of the same membrane were incubated either with anti-ANP antibody adsorbed with 10 nM ANP or with normal rabbit serum instead of primary antibody. Scale bar in B-E represents 50 μm. Sections counterstained with Mayer's Hematoxylin.