Table 2 of
Garcia-Hoyos, Mol Vis 2004;
10:426-431.Table 2 of
Garcia-Hoyos, Mol Vis 2004;
10:426-431.
Table 2. Primers for the amplification of SFRP1
Primers for the amplification of SFRP1 gene fragments. Fragment 1out contains the whole exon 1, and is the first step of a nested PCR, needed to amplify fragment 1A. "F" refers to a forward primer; "R" refers to a reverse primer.
Size
Fragment (bp) Primers
-------- ---- ----------------------------------
1out 1176 F: 5' GGGTGTTGAGCCGCGTCTGGTTCTA 3'
R: 5' ACGCGCTTAGGAATCACGTGCACAG 3'
1A 288 F: 5' CCGGGAGCTGATTGGCTGCG 3'
R: 5' GACCTCCGGGGACAAAAGGC 3'
1B 245 F: 5' GCCTTTTGTCCCCGGAGGTC 3'
R: 5' GCTCTGGTACGGGCCGATG 3'
1C 292 F: 5' GACTACGTGAGCTTCCAGTCGGAC 3'
R: 5' GCCAGCGACACGGGTAGATG 3'
1D 281 F: 5' GCTCTTCGCGCCCGTCTGCCTG 3'
R: 5' CGTAGGGTGGCGCGGGTTCTCC 3'
2 285 F: 5' CCAGAAGACCTTTAGCCACT 3'
R: 5' GCTGATGTATCTCGTTGCA 3'
3A 251 F: 5' CCCTCTTTTCTCTTCTTCTGTCCTC 3'
R: 5' GTACTGGCTCTTCACCTTGCG 3'
3B 251 F: 5' GTACTTGCTGACGGCCATCC 3'
R: 5' GCAAGAACAAGCCGACTGGA 3'
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