Figure 7. In vivo analysis of myocilin, C1/2/3/4/5FL and glaucoma associated mutations Arg82Cys and Cys433Arg

Western analysis of myocilin and myocilin mutants in media and cell lysate obtained 48 h following transfection of trabecular meshwork cells (TM5; gift from Abe Clark, Ph.D., Alcon Labs, Fort Worth, TX). A: Samples were separated on denatured, reduced SDS-PAGE gels (7.5%). B: Samples were separated on denatured, non-reduced SDS-PAGE gels (4-15% gradient).