Figure 4. Myocilin forms disulfide bonds in human aqueous humor

Equal volumes of aqueous humor was separated on SDS-PAGE gels under A: denatured, non-reduced conditions (75 μl) or B: denatured, reduced conditions (40 μl). Proteins were transferred to PVDF membrane and probed with anti-myocilin antibodies. Samples 1, 2, and 3 were obtained from patients undergoing cataract surgery. Sample 4 was collected from a glaucoma patient during trabeculectomy. Arrows indicate myocilin complexes.