Figure 6. Effects of scanning alanine mutagenesis of arrestin loop 248-253 on binding to R*P

Single alanine substitutions were made for each of the amino acids in loop 248-253 plus Tyr-254 in the adjacent βstrand. Arrestin bound to R*P in disc membranes was compared to native arrestin; error bars represent the standard error of the mean (n=3).