Figure 4 of
Smith, Mol Vis 2004;
Figure 4. Binding of AlexaFluor-594 labeled arrestin to rhodopsin
Labeled arrestins (4 μM) were mixed with phosphorylated rhodopsin (7 μM) in disc membranes; reactions were either kept in the dark or exposed to light for 2 min. Bound fractions were separated on 12% SDS PAGE and irradiated with ultraviolet light (A) or stained with coomassie brilliant blue (B).