Table 2 of
Wahlin, Mol Vis 2004;
10:366-375.Table 2 of
Wahlin, Mol Vis 2004;
10:366-375.
Table 2. Expression of molecular markers and housekeeping genes in photoreceptor and Müller cells
The relative intensity of the PCR products from cells selected by morphological criteria were evaluated using a scale ranging from (-) for absence of product to (+++) when the bands had maximum brightness in gels. Individual cells expressed cell markers corresponding to transcripts typical of those cell types. Housekeeping genes were also detected in these cells.
PhR Müller
---------------------------- ----------------------------
Genes - +/- + ++ +++ - +/- + ++ +++
----------- ---- --- --- --- --- ---- --- --- --- ---
PhR markers
β-PDE 41% 0% 3% 17% 38% 100% 0% 0% 0% 0%
GCAP1-α 41% 0% 0% 7% 52% 100% 0% 0% 0% 0%
Phosducin 3% 0% 11% 86% 0% 90% 0% 5% 5% 0%
Recoverin 69% 0% 14% 7% 10% 100% 0% 0% 0% 0%
Rhodopsin 0% 0% 0% 14% 86% 100% 0% 0% 0% 0%
Rom-1 21% 0% 3% 45% 31% 76% 0% 19% 5% 0%
S-antigen 11% 0% 22% 30% 38% 86% 0% 10% 0% 5%
Müller markers
CAC 100% 0% 0% 0% 0% 24% 5% 71% 0% 0%
CRALBP 95% 3% 3% 0% 0% 16% 0% 0% 0% 84%
GS 97% 0% 3% 0% 0% 6% 0% 0% 0% 94%
Vimentin 93% 0% 3% 3% 0% 35% 0% 12% 0% 53%
Housekeeping genes
β-actin 69% 0% 10% 10% 10% 40% 0% 0% 10% 50%
GAPDH 3% 0% 10% 83% 3% 5% 0% 19% 14% 62%
Ubiquitin C 62% 0% 5% 33% 0% 53% 0% 6% 41% 0%
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Relative intensity scale for PCR bands:
