Table 1 of
Wahlin, Mol Vis 2004;
10:366-375.Table 1 of
Wahlin, Mol Vis 2004;
10:366-375.
Table 1. Oligonucleotides sequences for PCR analysis
Oligonucleotide sequences corresponding to genes preferentially expressed in photoreceptors and Müllercells, to housekeeping genes, and to neurotrophic factor receptors. The oligonucleotides were designed against 3' regions of these genes.
Genebank
accession mRNA Amplicon Amplicon
Gene number length Forward primer Reverse primer region size (bp)
----------- --------- ------ -------------------------- -------------------------- ------------ ---------
β-PDE X55968 2743 GCCCTGGAGGAAGAAAAGAA AGGCAGAGTCCGTATGCAGT 2457 to 2630 174
GCAP1-α NM_008189 644 TAATCCCTGGAGCGACTCAT GTTCGCCGTTCTGGAGTCT 366 to 562 197
Phosducin NM_024458 1263 AGCAGAGGAGGTGGGTAATG GCCCAAATAAAAATGCCAAG 1079 to 1185 107
Recoverin NM_009038 1062 GCAAAAATGAAGTGCTGGAGA CAAACTGGATCAGTCGCAGA 451 to 663 213
Rhodopsin NM_145383 3249 GATGGAGCCTCAGATGTGGAGT ATCTAGCCAGCCTGAAGTGGAG 2971 to 3217 247
Rom-1 NM_009073 1349 GCTGAGGCCTAGAAACCTGA TCTCAACCTTGGTGGTTTCC 1081 to 1283 203
S-antigen NM_009118 1505 ATCCAGTGAAGTGGCTACCG GGAGGAATGCTCATGCTTG 1150 to 1439 290
CAC K00811 1490 TTAGTGACTTTTGATTTCTAGAGGTG TCCATTAGGTTATGGTAAGTTATCTG 1348 to 1451 104
CRALBP NM_020599 1869 ACTCTGTCCAGGGTGGAGGT CTGAGAGTGTTCGGGGAAGA 1698 to 1847 150
GS NM_008131 2731 AGTAAACACACCCCCACCTC GCCTGGCAGTTACAGTCTGTTT 2495 to 2665 171
Vimentin X56397 1748 CCTCTGGTTGACACCCACT CTGCAGTAAAGGCACTTGAAA 1378 to 1581 204
β-actin X03672 1892 AGGTGACAGCATTGCTTCTG GCTGCCTCAACACCTCAAC 1660 to 1856 197
GAPDH M32599 1228 TGGCCTCCAAGGAGTAAGAA GTGGGTGCAGCGAACTTTAT 1032 to 1228 197
Ubiquitin C NM_019639 498 AGAAAGAGTCCACCCTGCAC TCACACCCAAGAACAAGCAC 185 to 381 197
Trk B X17647 4351 GTGATGGGACTTGTGCCTTT ACCTGCCCTAGGCTGCTT 4183 to 4342 160
CNTFRα NM_016673 1975 CACCAAAGACCCCTCTCATC GCAAAGGTGGAAGGACTGAA 1511 to 1755 245
FGFR1 BC033447 2877 GCCGTGAGGTTTCTGTTTAGG CAGTCTCTGCCTCCCTGTCT 2618 to 2759 142
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